![]() ![]() The patients in this group are generally younger and often lack the traditional risk factors for head and neck squamous carcinoma (HNSCC) such as a history of smoking and alcohol abuse. In Sweden, the increase has been close to 5% per year between 19 which represents a doubling in incidence every 14 years. In recent years, there has been an increase in the incidence of oropharyngeal squamous cell carcinoma (OSCC) including cancers in the tonsils and the base of the tongue. This is an important step towards efficient treatment development. We established an in vitro tonsil carcinoma cell line containing episomal HPV16. The cell line was inhibited to a maximum of 18% by cetuximab. ![]() The ID50 for radiation was 0.90 Gy and the IC50 for cisplatin was 0.99 μmol/L. The karyotype was determined and the cell line demonstrated wild type TP53. The viral expression manifested 1.0 (CV% 0.1) E7 mRNA copies per HPV16 genome. In addition, HPV16 mRNA specific for the intact E2 gene was detected. Episomal HPV16 was present with an E2/E7 ratio of 1.1 (CV% 2.6). ![]() The complete HPV16 genome sequence was obtained. It contained 19.5 (CV% 3.7) HPV16 copies/cell (passage 8). The tumor was from a 48 year old non-smoking man with non-keratinizing, p16 positive tonsil OSCC, stage T2N0M0 with HPV16. ResultsĪfter 19 months (eight passages), one cell line, LU-HNSCC-26, was established in vitro and also grew as xenografts. In vitro studies of sensitivities to radiation, cisplatin and cetuximab were performed. The established cell line was analyzed for viral characteristics, cell karyotype, TP53 status, and growth capabilities in nude mice. Methodsįresh samples from 23 HPV-positive tonsil carcinomas were cultivated in vitro. The aim was to establish cell lines from HPV-positive tonsil carcinomas to be used for treatment development. Human papillomavirus (HPV) is an established risk factor for oropharyngeal squamous cell carcinoma (OSCC). ![]()
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